Oculus Quest 2 Controller not Working in My Unity Project

I'm currently in my final year and my final year project is to build VR game by using Unity version 2020.3.23. I'm already finish the game and using XR interaction toolkit, however I meet some problem :

1) The hand of my controller disappear when run the project in unity. Before, the hand appear like usual. 

2) After Build and Run the VR game in Oculus Quest 2, the controller not detected at all. However, when I testing the game in unity, it can run like usual and there's nothing error in my coding or project.

Did anyone know how to solve this problem?

Thanks in advance